Thermo Fisher, PV4663, LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15130:The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
In the LanthaScreen™ ERR alpha TR-FRET Coactivator Assay, ERR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50from a dose response curve of the compound. Based on the biology of the ERR alpha-coactivator peptide interaction, this ligand EC50is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).
Contents and Storage:The LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit contains ERR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Gene ID (Entrez): 2101
Ligand: ERR alpha
Packaging: 384-well Plate
Product Type: TR-FRET ERR Alpha Coactivator Assay Kit
Quantity: 800 x 20 μL Assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: ESRRA, NR3B1, ERR1, ERR alpha
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each