BRAND / VENDOR: Thermo Fisher

Thermo Fisher, PV4685, LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit, goat

CATALOG NUMBER: PV4685

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Product Description

LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15134:The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) delta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR delta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR delta ligand-binding domain (PPAR delta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
When running the LanthaScreen™ TR-FRET PPAR delta Coactivator Assay in agonist mode (to identify agonist compounds), PPAR delta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-labeled coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR delta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).
Contents and Storage:The LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit contains PPAR delta-LBD (GST) protein, fluorescently labeled C33 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C or 4°C).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Gene ID (Entrez): 5467
Ligand: PPAR delta
No. of Assays: 800 x 20 μL assays
Packaging: 384-well Plate
Product Type: TR-FRET PPAR Delta Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: PPAR delta, NR1C2, PPARD
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each

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