Thermo Fisher, PV4687, LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:The LanthaScreen™ TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.
To Assay:When performing the LanthaScreen™ TR-FRET TR alpha coactivator assay, TR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50from a dose response curve of the compound. Based on the biology of the TR alpha-coactivator peptide interaction, the ligand EC50is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).
Contents and Storage:The LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit contains TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Ligand: TR alpha
No. of Assays: 800 x 20 μL
Packaging: 384-well Plate
Product Type: TR-FRET TR Alpha Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: THRA, TR alpha, NR1A1
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each