Thermo Fisher, PV4688, LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.
When running the LanthaScreen™ TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Ligand: PAR beta
No. of Assays: 800 x 20 μL Assays
Packaging: 384-well Plate
Product Type: TR-FRET RAR Beta Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: RAR beta, NR1B2, RARB
Conjugate: Tb (Terbium), FITC (Fluorescein)
Product Line: LanthaScreen™
Unit Size: Each