Thermo Fisher, R0393, IPTG, dioxane-free

25 g Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates thelacrepressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of thelacoperon (seeReference 1). It is used in conjunction withX-Gal(#R0941) to determine thelacphenotype in blue/white colony screening.
Applications
• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (seeReference 1)
• Induction of gene expression under control of thelacpromoterNote
• Preparation of a 100 mM (23.
83 mg/mL) stock solution in water is recommended
• For blue/white colony screening use 0.
1 mM final IPTG concentration in LB (Luria Broth) media
• IPTG, dioxane-free, can be stored at +4°CChemical Name or Material: Isopropylthiogalactoside
Product Type: IPTG Solution
Quantity: 5 x 5 g
Concentration: 0.
1 mM
Form: Powder
Unit Size: Each