Thermo Fisher, R1171, IPTG Solution, ready-to-use

IPTG Solution, ready-to-use Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates thelacrepressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of thelacoperon. It is used in conjunction withX-Gal(#R0941) to determine thelacphenotype in blue/white colony screening.
Applications
• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
• Induction of gene expression under control of thelacpromoterNote
• For blue/white colony screening use 0.
1 mM final IPTG concentration in LB (Luria Broth) media
• Store IPTG Solution at -20°C.
Chemical Name or Material: Isopropylthiogalactoside
For Use With (Application): Protein Expression
Product Type: IPTG Solution
Quantity: 10 x 1.
5 mL
Concentration: 0.
1 M
Form: Liquid
Unit Size: Each