Thermo Fisher, R14207, Rhod-5N, Tripotassium Salt, cell impermeant

Rhod-5N, Tripotassium Salt, cell impermeant Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+release generated by photoreceptors and photoactivatable chelators. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette, microinjection, or ourInflux™ pinocytotic cell-loading reagent. The fluorescence signal from these cells is generally measured using fluorescence microscopy.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›Calcium Indicator (Cell-Impermeant Salts) Specifications:
• Label (Ex/Em of Ca2+–bound form): Rhod-5N (551/576 nm)
• Fluorescence intensity increase upon binding Ca2+: >100 fold
• Kdfor Ca2+in the absence of Mg2+, in buffer: ∼320 μM
• Exhibit fluorescence increase upon binding Ca2+with little shift in wavelengthUsing TPEN to Control Heavy Metal CationsIn addition, BAPTA-based indicators such as these bind various heavy metal cations (e.
g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelatorTPEN.
More Choices for Fluorescent Calcium IndicatorsWe offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, reviewFluorescent Ca2+Indicators Excited with Visible Light—Section 19.
3in the Molecular Probes™ Handbook.
For UV-excitable Ca2+indicators, protein-based Ca2+indicators, conjugates of Ca2+indicators, and for fluorescence-based indicators of other metal ions (i.
e., Mg2+, Zn2+) reviewIndicators for Ca2+, Mg2+, Zn2+and Other Metal Ions—Chapter 19in the Molecular Probes™ Handbook.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Detection Method: Fluorescence
Dye Type: Fluorescent Dye-Based
Quantity: 500 μg
Shipping Condition: Room Temperature
For Use With (Equipment): Fluorescence Microscope
Product Type: Calcium Indicator
Unit Size: Each