Thermo Fisher, R37167, CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

Thermo Fisher, R37167, CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Exceptionally bright green fluorescence upon binding to nucleic acids
• Rapid staining of apoptotic cells without wash stepsCellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 106cells, incubate, and analyze.
CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
Color: Green
Excitation/Emission: 504/523
Label Type: Other Label(s) or Dye(s)
Label or Dye: CellEvent™ Caspase 3/7 Green, SYTOX AADvanced Dead Cell Stain
Product Line: CellEvent™ Ready Flow
Product Type: Caspase 3/7 Reagent
Quantity: 120 assays
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus
Format: Dropper Bottle
Unit Size: Each