Thermo Fisher, R37606, NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)

NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue Fixed Cell ReadyProbes Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Also available:DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)at 12. 5X concentration (5 μM) in water.
• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• DAPI is excited with UV light and detected through a blue/cyan filterSee other ReadyProbes reagents for cell stainingSee other nuclear stains for imagingCell imaging applicationsDAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1). . Suggestions for useIn most cases 2 drops/ml of NucBlue Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine Alexa Fluor 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue Fixed Cell Stain is ideal for use with antibody-based applications. Visualize staining your cell without wasting your reagents, antibodies, or time with our newStain-iT Cell Staining Simulator. Color: Blue
Detection Method: Fluorescence
Dye Type: DAPI
Emission: Visible
Excitation Wavelength Range: 360⁄460
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Form: Liquid
Product Line: Molecular Probes™
Quantity: 6 vial(s) kit
Shipping Condition: Room Temperature
Label Type: Fluorescent Dye
Product Type: Nucleic Acid Stain
SubCellular Localization: Nucleus
Unit Size: Each