Thermo Fisher, R37609, ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green ReadyProbes® Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue® Live reagent (Hoechst 33342) and NucGreen® Dead reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue® Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen® Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set. This kit is appropriate for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.
NucBlue® Live reagent:stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)NucGreen® Dead reagent:stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523 nm)See other ReadyProbes® reagents for cell stainingLearn more about other assays for cell viabilitySuggestions for use
• NucBlue® Live and NucGreen® Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Cell Type: Mammalian Cells, Eukaryotic Cells
Description: ReadyProbes Cell Viability Imaging Kit, Blue/Green
Detection Method: Fluorescence
Dye Type: 3 x 2.
5 mL NucBlue™ Live
Format: Dropper bottles
Quantity: 1 kit
Shipping Condition: Room Temperature
Color: Blue, Green
Emission: 360/504
Excitation Wavelength Range: 460, 523 nm
For Use With (Application): Viability Assay
For Use With (Equipment): Fluorescence Microscope
Product Line: ReadyProbes
Product Type: Cell Viability Imaging Kit
Unit Size: Each