Thermo Fisher, S21377, Streptavidin, Alexa Fluor™ 660 conjugate

Streptavidin, Alexa Fluor™ 660 conjugate Alexa Fluor™ 660 streptavidincomprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.
5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor™ 660 Streptavidin Conjugates:
•Alexa Fluor™ 660 streptavidin conjugate has Ex/Em maxima of ∼ (663/690)
•Bright, photostable fluorescence
•High solubility in aqueous solutions
•Available in multiple colors
•Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Alexa Fluor™ DyesAlexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibitimproved photostabilityandbrightnessand improved solubility in aqueous solutions. Available in abroad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous BiotinNaturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try ourEndogenous Biotin-Blocking Kitto minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:Learn more about Avidin-Biotin DetectionLearn more about Alexa Fluor™ DyesFind out about other Labeled Streptavidin ConjugatesRead Avidin and Streptavidin Conjugates-Section 7.
6in the Molecular Probes HandbookLabel or Dye: Alexa Fluor Dyes
Product Type: Streptavidin Conjugate (fluorescent)
Quantity: 1 mg
Shipping Condition: Room Temperature
Conjugate: Alexa Fluor 660
Form: Solid
Product Line: Alexa Fluor™
Unit Size: Each