Thermo Fisher, V19020, pPICZ A, B, & C Pichia Vectors

pPICZ A, B, & C Pichia Vectors The pPICZ A, B, & CPichiaVectors are designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein. These vectors contain the Zeocin™ resistance gene for direct selection of multi-copy integrant strains. By selecting with increasing amounts of Zeocin™, strains with multiple copies of your gene of interest integrated into the genome are obtained. Increasing the number of copies of the gene of interest in a recombinantPichiastrain can result in higher expression levels. The vectors are included in the EasySelect™ Pichia Expression Kit (Cat. No. K1740-01).
Features of the pPICZ vectors include:
• InducibleAOX1promoter for high-level expression inPichia pastorisc-mycepitope tag for convenient detection with an Anti-mycAntibody
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Zeocin™ resistance for direct selection of multi-copy integrantsAntibiotic Resistance Bacterial: Zeocin™ (ZeoR)
Product Type: Pichia Expression Vector
Quantity: 20 μg
Vector: pPIC
Cloning Method: Restriction Enzyme/MCS
Promoter: AOX1
Protein Tag: His Tag (6x), c-Myc Epitope Tag
Unit Size: 20 µg