Thermo Fisher, V35120, pRSET A, B, & C Bacterial Expression Vectors

pRSET A, B, & C Bacterial Expression Vectors The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3)E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes. The pRSET vector offers:
• Bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection with the Anti-Xpress™ Antibody
• Enterokinase cleavage site for removal of fusion tagA set of three vectors is provided (A, B, and C). Each has the N-terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Product Type: Bacterial Expression Vector
Quantity: 20 μg
Selection Agent (Eukaryotic): None
Vector: pRSET
Cloning Method: Restriction Enzyme/MCS
Promoter: T7
Protein Tag: His Tag (6x)
Unit Size: 20 µg