Thermo Fisher, V37006, Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector

Vivid Colors™ pLenti6.
3/V5-GW/EmGFP Expression Control Vector The Vivid Colors™ pLenti6.
3/V5-GW/EmGFP Expression Control Vector is a ViraPower™ HiPerform™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ HiPerform™ Lentiviral Expression Systems as a positive control to enable the detection of higher levels of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the SV40 promoter for driving expression of the blasticidin stable selection marker. The vector is equipped with two key genetic elements, making them Hiperform™ vectors: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in EmGFP expression compared to vectors lacking these elements. This control vector is not designed for generating EmGFP fusion proteins and does not express the V5 epitope.
AdvantagesLentivirus based expression of EmGFP in dividing and non-dividing mammalian cellsServe as a quick positive control for transfection and lentiviral productionServe as a quick titer control in determination of lentivirus titerKey FeaturesConstitutive expression with CMV promoterHigh level expression of EmGFP without V5 epitopeWPRE and cPPT sequences produce at least 4-fold increase in EmGFP expression compared to other Lenti vectors without these elementsBlasticidin selection marker for stable selectionKit IncludespLenti6.
3/V5-GW/EmGFP Expression Control VectorRelated SKUsViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit (Cat. No. K531000)ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K533000)ViraPower™ HiPerform™ T-Rex™ Gateway™ Expression System (A11141)ViraPower™ HiPerform™ Promoterless Gateway™ Expression System (A11145)Constitutive or Inducible System: Constitutive
Delivery Type: Lentiviral
For Use With (Application): Reporter Assays, Viral Expression
Product Type: Lentiviral Expression Vector
Quantity: 20 μg
Reporter Gene: GFP (EmGFP)
Selection Agent (Eukaryotic): Blasticidin
Vector: pLenti
Cloning Method: Gateway
Product Line: ViraPower™, Vivid Colors™
Promoter: CMV
Protein Tag: V5 Epitope Tag
Unit Size: 20 µg