Thermo Fisher, V43001, pBAD/His Kit

pBAD/His Kit The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides:
• ThearaBADpromoter for tightly regulated expression
• Translation initiation signals optimized forE. coliexpression
• N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody
• Enterokinase cleavage site for removing the N-terminal tag following purificationThree vectors are provided (A, B, and C). Each has the N-terminal tag in a different readingframe relative to the multiple cloning site to simplify in-frame cloning of your gene.
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: Arabinose
Product Type: Bacterial Expression Vector
Quantity: 1 kit
Selection Agent (Eukaryotic): None
Vector: pBAD
Cloning Method: Restriction Enzyme/MCS
Promoter: araBAD
Protein Tag: His Tag (6x)
Unit Size: 1 kit