BRAND / VENDOR: Thermo Fisher

Thermo Fisher, V44001, pBAD/Myc-His Kit

CATALOG NUMBER: V44001

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Product Description

pBAD/Myc-His Kit The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:
• ThearaBADpromoter for tightly regulated expression
• Translation initiation signals optimized forE. coliexpression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminalc-mycepitope for detection and analysis with an Anti-mycAntibodyThree vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: Arabinose
Product Type: Bacterial Expression Vector
Quantity: 1 kit
Selection Agent (Eukaryotic): None
Vector: pBAD
Cloning Method: Restriction Enzyme/MCS
Promoter: araBAD
Protein Tag: c-Myc Epitope Tag
Unit Size: 1 kit

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