Thermo Fisher, V45001, pBAD/gIII Kit

pBAD/gIII Kit The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of thearaBADpromoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into theperiplasmic space. The vector provides:
• ThearaBADpromoter for tightly regulated expression
• Translation initiation signals for optimizedE. coliexpression
• Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminalc-mycepitope for detection and analysis with an Anti-mycAntibodyA set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Constitutive or Inducible System: Inducible
Inducing Agent: Arabinose
Product Type: Bacterial Expression Vector
Quantity: 1 kit
Selection Agent (Eukaryotic): None
Vector: pBAD
Cloning Method: Restriction Enzyme/MCS
Promoter: araBAD
Protein Tag: c-Myc Epitope Tag
Unit Size: 1 kit