Thermo Fisher, V79520, pcDNA™3.1 (-) Mammalian Expression Vector

pcDNA™3.
1 (-) Mammalian Expression Vector This pcDNA™3.
1(-)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin™ selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.
1 Expression Vector FamilyThree untagged versions of pcDNA™3.
1 (available separately), each with a different selectable marker (Geneticin™, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.
e.,COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance inE. coliDelivery Type: Transfection
For Use With (Application): Constitutive Expression
Product Type: Mammalian Expression Vector
Quantity: 20 μg
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: pcDNA
Cloning Method: Restriction Enzyme/MCS
Product Line: pcDNA™
Promoter: CMV
Protein Tag: Untagged
Unit Size: 20 µg