Thermo Fisher, V90020, pSecTag2 A, B, & C Mammalian Expression Vectors

pSecTag2 A, B, & C Mammalian Expression Vectors pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:
• Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
• Cytomegalovirus (CMV) promoter for high-level constitutive expression
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• C-terminal c-mycepitope for detection with an Anti-mycAntibody
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.
g.,COS-1, COS-7)The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used inE. coli.
The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
Delivery Type: Transfection
For Use With (Application): Secreted Expression
Product Type: Mammalian Expression Vector
Quantity: 20 μg
Selection Agent (Eukaryotic): Zeocin™
Vector: pSec
Cloning Method: Restriction Enzyme/MCS
Promoter: CMV
Protein Tag: His Tag (6x), c-Myc Epitope Tag, IgK Leader Sequence
Unit Size: 20 µg