Waters, 186003950, ACQUITY UPLC Oligonucleotide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 30K, 1/pk

Waters Oligonucleotide Separation Technology (OST) columns are used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography. Each column is QC tested using Waters MassPrep OST standards to ensure repeatable performance for your most demanding oligonucleotide assays.acqui

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 30000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Oligonucleotide
Format: Column
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 18 %
UNSPSC: 41115709
Application: Oligonucleotide, Gene Therapeutics
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about ACQUITY UPLC Oligonucleotide BEH C18 Column What type of applications is the ACQUITY UPLC Oligonucleotide BEH C18 Column designed for? The ACQUITY UPLC Oligonucleotide BEH C18 Column is tailored for the high-resolution separation of synthetic oligonucleotides, including siRNA and DNA aptamers, essential for therapeutic development and genetic research. How does the ACQUITY UPLC Oligonucleotide BEH C18 Column achieve high-resolution separation? The column's small particle size (1.7µm) and BEH technology provide enhanced mass transfer and column efficiency, which translate to sharper peaks and better resolution in oligonucleotide separations. Can the ACQUITY UPLC Oligonucleotide BEH C18 Column be used for the analysis of modified oligonucleotides? Yes, the robust C18 phase and controlled surface chemistry are designed to handle the separation of modified oligonucleotides, including those with labels or conjugates. What are the advantages of using the ACQUITY UPLC Oligonucleotide BEH C18 Column in regulatory environments? The ACQUITY UPLC Oligonucleotide BEH C18 Column offers reliability and reproducibility, which are crucial for meeting regulatory requirements in pharmaceutical and biotechnological applications. How should the ACQUITY UPLC Oligonucleotide BEH C18 Column be cleaned and stored? The column should be cleaned with a series of washes using suitable solvents to prevent the build-up of matrix components and stored dry or in a recommended storage solvent as per the manufacturer's instructions to maintain its performance.

FAQ
What Are Oligonucleotides? Oligonucleotides are short DNA or RNA molecules, oligomers, with a wide range of applications including genetic testing, research, and forensics. They are commonly made in the laboratory using solid-phase chemical synthesis. They are also found in nature as small RNA molecules that function in the regulation of gene expression. Oligonucleotides are characterized by the sequence of nucleotide residues that make up the entire molecule, with the length usually denoted by the suffix “-mer.”

Chromatography is one analytical method for the separation of oligonucleotides; mass spectrometry can be used for oligonucleotide analysis. Procedures that use oligonucleotides may include DNA microarrays, ASO analysis, and the synthesis of artificial genes.