Waters, 186004869, XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2-11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk Based on Waters’ novel ethylene bridged hybrid (BEH) particle, XBridge BEH Amide Columns utilize a chemically stable, trifunctionally bonded amide phase to enable a new level of stability and versatility for HILIC separations. With the same selectivity as ACQUITY UPLC BEH Amide columns, scientists can easily transfer separations between HPLC and UPLC lab equipment as well. XBridge BEH Amide Columns were designed specifically to overcome common challenges in retaining and separating extremely polar compounds and metabolites that are too polar to be retained by reversed-phase chromatography. The columns facilitate the use of a wide range of mobile phase pH (2 – 11) in order to facilitate this exceptional retention of polar analytes that spans a wide range of polarity, structural moiety, and pKa. Along with enhanced retention of polar compounds, XBridge BEH Amide columns offer an increased mass spectrometry response, direct compatibility with sample preparation eluates like PPT, LLE, and SPE, and orthogonal selectivity when compared to reversed-phase materials. XBridge BEH Amide Columns are well suited to a range of applications, including the analysis of carbohydrates. The 3.5 µm particle size enables high resolution, high-speed analysis of carbohydrates in complex sample matrixes while maintaining or improving chromatographic resolution. In addition to this, increased chemical stability allows for the use of high pH and high temperature to collapse anomers without the loss of reducing sugars. Unlike amine-based columns for this application, these columns are not susceptible to Schiff base formation, allowing for improved quantitation accuracy Before use in carbohydrate analysis, scientists can benchmark separations using HILIC QC Reference Material .

FAQ
What Are the Operating Limits For XBridge BEH Amide Columns? The recommended pH limit for XBridge BEH Amide Columns includes a wide range of 2 – 11.
These columns can tolerate pressures of up to 6,000 psi (400 bar or 40 Mpa). However, pressures greater than 4,000 to 5,000 psi should be avoided when possible to maximize column and system lifetimes.
XBridge BEH Amide Columns have a recommended temperature limit of 90 degrees C in order to enhance selectivity, lower solvent viscosity, and increase mass transfer rates. However, temperatures above ambient will have a negative effect on lifetime, which can vary based on the pH and bugger conditions used.