Waters, 186005657, ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 75 mm, 1K - 30K, 1/pk

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 30000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 2.1 mm
Length: 75 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115709
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 75 mm, 1K - 30K, 1/pk Based on the chemically stable, trifunctionally bonded amide phase of the BEH particle, ACQUITY UPLC BEH Amide Columns enable a new dimension in stability and versatility. Waters’ focus on innovative lab equipment and stationary phases for HILIC (Hydrophilic Interaction Chromatography) has allowed this column to overcome the challenge of retaining and separating extremely polar compounds through use of the novel ethylene bridged hybrid particle. ACQUITY UPLC BEH Amide Columns enable exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa through the use of a wide range of mobile phase pH, from 2 to 12. Along with the enhanced retention of polar analytes and metabolites, the column offers mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), and orthogonal selectivity when compared to other reversed-phase materials. Analysis of carbohydrates, including monosaccharides, disaccharides, oligosaccharides, and polysaccharides, is improved through ACQUITY UPLC BEH Amide Columns’ use of BEH particles. This technology offers increased chemical stability that enables the use of both high pH and high temperature to collapse, reducing sugar anomers, shortening the analysis time, and improving MS detection. Assay robustness is also improved through the exceptional column lifetime provided by the BEH particle. Lifetime can be further improved through the use of ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk . Unlike amine-based columns commonly used in carbohydrate analysis, the ACQUITY UPLC BEH Amide column is not vulnerable to Schiff-based formation, allowing for improved quantitation accuracy.

FAQ
What Are Carbohydrates? Carbohydrates are any molecules that contain carbon, hydrogen, and oxygen, where there are twice as many hydrogen atoms as carbon or oxygen atoms. There are a variety of forms of carbohydrates used in analysis.
Monosaccharides are simple sugars in which there is one oxygen atom and two hydrogen atoms for each carbon atom present in the molecule; disaccharides are made up of two monosaccharides joined together through a condensation reaction. Polysaccharides then combine more than two monosaccharide molecules through condensation reactions.
Molecules with 3 to 10 sugar units are oligosaccharides, while molecules with 11 or more are true polysaccharides.