Waters, 186006031, XBridge BEH C18 Column, 130Å, 2.5 µm, 2.1 mm X 100 mm, 1/pk

As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 18 %
eCord: Yes
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH C18 XP Column, 130Å, 2.5 µm, 2.1 mm X 100 mm, 1/pk With the flexibility to work over an unparalleled range of mobile phase pH and temperature while providing unmatched column lifetime, XBridge BEH C18 XP Columns allow for maximum flexibility in HPLC methods development. Chromatographers choose XBridge columns to develop robust separations in less time and with greater confidence, harnessing the full power of pH. With the combination of cutting-edge bonding techniques, end-capping, and particle synthesis with the industry’s best manufacturing, these columns will be a solution you can trust in challenging separations. As a part of the XBridge XP 2.5 µm Column family, this column allows for the optimization of the total cycle time without sacrificing resolution. This technology improves existing HPLC productivity by 2 to 4 times, with unmatched selectivity and flexibility. Directly scalable to 1.7 µm ACQUITY UPLC BEH Columns and larger 3.5/5 µm XBridge HPLC Columns, they are designed to withstand higher pressures of 18,000 psi. Ethylene Bridged Hybrid (BEH) Technology synthesis is the basis of this lab equipment , ensuring extreme column performance and long column lifetime, even under harsh operating conditions. Prepared from two high purity monomers, BEH results in a highly stable, pH resistant, and mechanically strong particle. Paired with the trifunctionally bonded C18 ligand, the XBridge BEH C18 XP column is a universal option for HPLC separations, leading to the widest usable pH range, superior low pH stability, and ultra-low column bleed. Laboratories can perform further testing using Reversed-Phase QC Reference Material . XBridge XP 2.3 µm Columns will exhibit maximum chromatographic performance when used with other tools from the ACQUITY UPLC System family.

FAQ
How Should I Prepare Samples for Use With XBridge BEH C18 XP Columns? It is recommended to prepare the sample in the initial mobile-phase conditions or a weaker solvent in order to achieve the best peak shape and sensitivity. If the sample is not prepared in the mobile phase, ensure that the sample, solvent, and mobile phases are miscible as this avoids sample and/or buffer precipitation.
Filter samples with a 0.2 µm membrane in order to remove particles. If the sample is dissolved in a solvent that contains an organic modifier, make sure the membrane/filter material is compatible with the solvents in use.