Waters, 186006041, XBridge BEH C8 Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk

The XBridge BEH C8 sorbent is less retentive compared to the XBridge BEH C18 sorbent. Anyone developing methods may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The trifunctionally bonded sorbent offers you the widest usable pH range (1-12), superior low pH stability, and ultra-low column bleed. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications
Chemistry: C8
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L7
Inner Diameter: 2.1 mm
Length: 50 mm
Carbon Load: 13 %
eCord: Yes
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH C8 XP Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk Bridge the gap between HPLC and UPLC technology with the 2.5 µm particle eXtended Performance XBridge columns, designed to enable exceptional separation performance, robustness, and throughput for HPLC assays. At the same time, the XBridge BEH C8 XP Column is compatible with all HPLC, UHPLC, and UPLC Technology platforms. By utilizing advanced packing technology, XBridge BEH C8 XP Columns are able to achieve exceptional performance and equivalent backpressure when compared to core-shell columns while exhibiting improved loading capacity and particle size scalability inherent to fully porous particle columns. XBridge BEH columns are designed to maximize productivity for method development by improving pH stability and column reliability while maximizing particle efficiency. By basing these columns on the Ethylene Bridged Hybrid (BEH) particle, column performance and lifetime are improved even under harsh operating conditions thanks to the unique combination of two high purity monomers. Tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE) combine to create a highly stable, pH resistant, and mechanically strong particle with applications in a range of lab equipment . The XBridge BEH C8 XP Column features XBridge BEH C8 sorbent, a less retentive option when compared with the XBridge BEH C18 sorbent. Method developers may prefer the speed offered by the less retentive C8 ligand, while not compromising peak shape or chromatographic performance. This trifunctionally bonded sorbent allows for the widest usable pH range (2-12) and ultra-low column bleed. All XBridge packing materials are designed to provide excellent peak shape, high efficiency, and excellent stability for both acidic and basic mobile phases. To ensure these results, each batch of material is tested chromatographically to ensure reproducible performance. Reversed-Phase QC Reference Material can be used for further testing.

FAQ
How Do I Test Column Efficiency In The XBridge BEH C8 XP Column? Before using your column for the desired application, you can perform a simple efficiency test. Waters recommends using a suitable solute mixture to analyze the column upon receipt. You can reference the Performance Test Chromatogram for guidance.
Then, determine the number of theoretical plates (N) and use this value for periodic comparisons. Repeat the test at predetermined intervals in order to track column performance.
Keep in mind that slight variations may be found between HPLC systems due to varying factors.