Waters, 186006089, XBridge BEH Amide Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 2.1 mm
Length: 50 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide XP Column, 130Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk Add the XBridge BEH Amide XP Column to your lab equipment collection in order to retain extremely polar compounds, including carbohydrates and sugars. The use of the XBridge Amide analytical column provides you with a chemically stable, trifunctionally bonded amide phase that is stable across a wide operating pH range of 2 to 11, making it possible for you to conduct the separation of polar analytes across a variety of features such as polarity, structural moiety, and pKa. The 2.5 µm Xbridge eXtended Performance [XP] column is packed in ultra-low dispersion hardware. This makes it possible to get exceptional separation performance, robustness, and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption. The product listed here is particularly suited for the analysis of carbohydrates with the 2.5 µm particle size and 130Å pore size, enabling high resolution and high-speed analysis in complex sample matrices while maintaining or improving the chromatographic resolution. The XBridge BEH Amide Column utilizes Waters’ novel ethylene bridged hybrid (BEH) particle, which makes use of a chemically stable, trifunctionally bonded amide phase to offer unprecedented stability and versatility in HILIC separations lab equipment. Waters has developed this ability as an innovative approach to overcoming the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. The XBridge BEH Amide Column has the same selectivity as ACQUITY UPLC BEH Amide columns. This enables scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms. Refer to our product catalog or online store to shop for lab equipment that is authentic and available at the best available rates in the market. Protect your analytical XBridge column and enhance its performance by using the XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk . The cartridge works by removing unwanted particles from the mobile stream.

FAQ
What Is The Relationship Between pKa and pH? The pH is a measure of the concentration of hydrogen ions in an aqueous solution. Essentially, pKa tells you what the pH needs to be in order for a chemical species to donate or accept a proton. The relationship between pH and pKa is described by the Henderson-Hasselbalch equation.