Waters, 186006505, ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm, 1/pk

Based upon the highly successful BEH particle technology, the  ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH125 SEC Protein Standard at no additional cost. (PN: 176003906)  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 10000 psi (690 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 80000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 125 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 150 mm, 1K - 80K, 1/pk Calculate the aggregate determinations for molecular weights between 1000- 80,000 Daltons faster than traditional SEC assays with the ACQUITY UPLC Protein BEH SEC Column. The analytical column has been optimized for the analysis of proteins and their aggregates to offer robust, fast, and accurate determinations. Get exceptional improvements in resolution, throughput, and sensitivity for assays with the ACQUITY UPLC Protein BEH SEC Column. The column enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC), thus increasing the efficacy since the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics. The packing materials used in the ACQUITY UPLC Protein BEH SEC Column were designed specifically for use with the ACQUITY UPLC System. The ACQUITY UPLC Protein BEH SEC Columns are manufactured in a cGMP, ISO 9001 certified plant using ultra-pure reagents. In addition to this, each batch of the packing material is tested chromatographically with acidic, basic, and neutral analytes. The results from these tests are held to narrow specification ranges to assure excellent, reproducible performance. Every ACQUITY UPLC Protein BEH SEC Column is individually tested and a Performance Chromatogram and Certificate of Batch Analysis are provided on the eCord Intelligent Chip so you can rely on the quality of your lab equipment and can be certain that you will get consistent results. Enhance the life of your analytical column by using the ACQUITY UPLC Protein BEH SEC Guard Column, 125Å, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pk . The Guard column has been designed to work specifically with the ACQUITY UPLC Protein BEH SEC Column and extends the lifetime of the column by removing any non-desired sample matrix or particulates onto the column without degrading the separation. It can be easily replaced to restore separation performance and extend the analytical column’s lifetime. Check our catalog to find all related products and other variants of the same listing as well as our complete range of products, and also to shop for lab equipment.

FAQ
What Is The pH Range For ACQUITY UPLC Protein BEH SEC Column? It is recommended that the ACQUITY UPLC Protein BEH SEC Column be operated in the pH range of 1–12. In addition to this, the column’s lifetime will vary depending upon the operating temperature, the type, and concentration of buffer used.