Waters, 186006506, ACQUITY UPLC Protein BEH SEC Column, 125 Å, 1.7 µm, 4.6 mm X 300 mm, 1/pkg

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 300 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note:  The same column is also available with the BEH125 SEC Protein Standard  at no additional cost. (PN: 176003907)  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 7000 psi (483 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 80000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 125 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 300 mm
Carbon Load: 12 %
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm X 300 mm, 1K - 80K, 1/pk With the ability to rapidly separate and accurately quantitate protein or peptide monomers from their respective aggregates, ACQUITY UPLC Protein BEH SEC Columns are chosen by biopharmaceutical chromatographers across the globe. These columns are enabled by Waters’ unique Ethylene-Bridged Hybrid (BEH) Diol coated particle technology, allowing accurate measurements of aggregation species up to 10 times faster and with less eluent than traditional SEC methods that use soft gels or >5µm rigid particles. This allows a significant reduction in the requirement for high salt concentration mobile phases to decrease non-desired, ionic secondary interactions between protein/peptide and SEC particle surface. As a well-established and USP/EP approved method for the analysis of undesired protein or peptide aggregates from active monomeric forms, SEC separations have traditionally used HPLC technology. By performing separations using Waters UPLC-based lab equipment , chromatographers can achieve comparatively improved component resolution while also reducing both analysis time and mobile phase consumption. This allows products to be moved to market faster while still offering the consistency required by drug regulatory bodies. The ACQUITY UPLC Protein BEH SEC Column and ACQUITY UPLC Protein BEH SEC Guard Column, 125Å, 1.7 µm, 4.6 mm X 30 mm, 1K - 80K, 1/pk have unique surface chemistry particles that significantly reduce secondary interactions common in SEC, leading to less need for aggressive mobile-phase salt concentrations. All ACQUITY UPLC SEC columns are quality control tested using relevant proteins and peptides to ensure superior consistency across batches and giving you increased confidence in proven and validated methods. All products are synthesized using high-quality raw materials and manufactured in ISO-certified manufacturing facilities.

FAQ
What Is The Difference Between A Peptide And A Protein? Both proteins and peptides are fundamental components of cells that carry out critical biological functions. Proteins give shape to cells and respond to signals transmitted from the surrounding environment, while peptides can play a key role in regulating other molecules’ activity. The two are very similar, both made up of chains of amino acids and held together by peptide bonds.
The main distinction is that peptides are much smaller than proteins, typically containing 2-50 amino acids, while proteins are made up of over 50 amino acids. Peptides also tend to have a less defined structure than proteins, which can adopt complex formations called secondary, tertiary, and quaternary structures.