Waters, 186006596, XBridge BEH Amide Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pk Since 2003, Waters has been committed to the development of innovative stationary phases for Hydrophilic Interaction Chromatography (HILIC) as a way to overcome the challenge of retaining and separating extremely polar compounds. Based on the novel ethylene bridged hybrid (BEH) particle technology, XBridge BEH Amide Columns make use of a chemically stable, trifunctionally bonded amide phase that enables a new dimension of stability and versatility for HILIC separations. With the same selectivity as ACQUITY UPLC BEH Amide columns, scientists can transfer separations between HPLC and UPLC technology platforms seamlessly. XBridge BEH Amide Columns are designed to retain polar analytes and metabolites that are too polar to retain with reversed-phase chromatography and facilitate the use of a wide range of mobile phase. This facilitates exceptional retention of polar analytes that span a wide range in polarity, structural moiety, and pKa. In addition to this enhanced retention, XBridge BEH Amide Columns offer increased mass spectrometry response, direct compatibility with sample preparation elutates, as well as orthogonal selectivity compared to reversed-phase materials. One of the most common classes of compounds analyzed by HILIC are carbohydrates, and XBridge BEH Amide Columns are a piece of lab equipment designed for this task. The increased chemical stability enables the use of high pH and high temperature to collapse anomers without the loss of reducing sugars and prevent Schiff base formations, improving quantitation accuracy. XBridge BEH Amide Columns are created using strict manufacturing processes that guarantee reproducibility, and testing can be performed using HILIC QC Reference Material from Waters.

FAQ
What Injection Solvents Should I Use With XBridge BEH Amide Columns? If possible, any injection solvents used should be as close to the mobile phase composition as possible (if isocratic) or the starting gradient conditions. A generic injection solvent is 75:25 acetonitrile:methanol, which is a good compromise between analyte solubility and peak shape.
When separating saccharides with limited solubility in organic solvents, higher concentrations of aqueous solvent in the sample are acceptable. 50:50 acetonitrile:water can offer satisfactory results.
Injection solvent’s influence on peak shape should be determined in an experimental manner. In some cases, injections of water may not have an adverse impact on peak shape.