Waters, 186006850, ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm, 1/pk

The ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm kit contains an advanced, ultra-low volume guard device (4.6 mm ID x 30 mm) containing BEH450, SEC, 2.5 µm particles and guard connection tubing (0.005” x 1.75”).

Specifications
Chemistry: SEC
Separation Mode: SEC/GPC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 3000 psi (207 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Guard Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 30 mm
Carbon Load: 9 %
eCord: No
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Guards
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm, 1/pk The resolution, throughput, and sensitivity of assays can all be significantly improved with the help of the ACQUITY UPLC Protein BEH SEC Column. The ACQUITY UPLC Protein SEC Column enables you to monitor the presence of protein aggregates by size-exclusion chromatography (SEC) which results in greater efficacy as the presence of these proteins can affect the safety and efficacy of biologic-based treatments. Delivering quick, precise aggregate determinations up to 10 times faster than conventional HPLC-based SEC procedures is simple with the inclusion of the ACQUITY UPLC Protein BEH SEC Column. The BEH particle technology was used to construct the column's core, which is fully tuned for the study of proteins and their aggregates with molecular weights between 100,000 and 1,500,000 Daltons. The analytical column has been developed for the analysis of proteins and their aggregates to provide reliable, quick, and accurate determinations. It is advised to utilize it in UPLC SEC applications with an adequate low LC system volume for maximum SEC separation performance. A sophisticated, ultra-low volume guard device (4.6 mm ID x 30 mm) comprising BEH450, SEC, 2.5 µm particles, and guard connection tubing (0.005" x 1.75") are included in this ACQUITY UPLC Protein BEH SEC Guard Column, 450, 2.5 µm, 4.6 mm x 30 mm package. The ACQUITY UPLC BEH SEC Guard Columns are produced at Waters manufacturing facilities and plants, which have gained a reputation as industry leaders and strictly adhere to cGMP and ISO 9001 standards and Operating procedures. You can visit the Waters website to shop for lab equipment and make sure you have everything you need. You can review the entire catalog of Waters product ranges from the website. We highly recommend also checking out the BEH450 SEC Protein Standard Mix, which is a five-component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil) used to benchmark ACQUITY UPLC BEH450 SEC, 2.5 µm Columns. It is frequently used in conjunction with the ACQUITY UPLC Protein BEH SEC Guard Column. Similar to the QCRM product line, it is advised that this standard be utilized frequently for testing column and system performance.

FAQ
How Do You Define Elution In Chromatography, And What Is Its Importance? Elution, a chromatographic procedure, refers to the separation of an antibody from its bound antigen by utilizing a solvent to extract the adsorbed material from a solid adsorbing media. The eluent or eluant, serving as the carrier component of the mobile phase, facilitates the movement of analytes through the chromatograph during the process.