Waters, 186006851, ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 150 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note: This column is also shipped with the BEH450 SEC Protein Standardat no additional cost at no additional cost (PN: 176002996) . Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: SEC
Separation Mode: SEC/GPC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 3000 psi (207 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 9 %
eCord: Yes
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 150 mm, 100K - 1M, 1/pk Based on Waters’ successful ethylene bridged hybrid (BEH) particle, the ACQUITY UPLC Protein BEH SEC Column is the first commercially available sub 2 µm column designed specifically for SEC chemistries. The columns have been designed and optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. This allows for accurate aggregations at up to ten times the speed of traditional HPLC-based SEC methods. The excellent performance of these columns is possible due to the BEH Diol coated particle technology, which allows for rapid separation and accurate quantitation of protein and peptide monomers from their respective aggregates. When chromatographers choose lab equipment based on this technology, they are able to feel confident in their measurements of aggregation species. Additionally, they can use less eluent than other SEC methods require and reduce the requirement for high salt concentration mobile phases. Many chromatographers note a decrease in non-desired, ionic secondary interactions between protein/peptide and SEC particle surfaces. All ACQUITY UPLC Protein BEH SEC Columns are quality tested with relevant proteins and peptides in order to maintain unmatched consistency and reproducibility. Manufacturing is done in a cGMP, ISO 9001 certified plant and uses ultra-pure reagents, with all results held to narrow specification ranges that ensure high quality and reproducibility. Every batch of packing material is tested and qualified using BEH450 SEC Protein Standard Mix . Each column has been individually tested and includes a Performance Test Chromatogram and a Certification of Analysis. Columns are sold individually and come one to a package.

FAQ
What Is SEC? SEC stands for size-exclusion chromatography, a chromatographic method based on the principle that particles of different sizes will elute (or filter) through a stationary phase at different rates, leading to the separation of a solution based on particle size or sometimes molecular weight. SEC is most commonly used with large molecules or macromolecular complexes, like proteins or industrial polymers.
Chromatographers choose SEC because of the good separation of molecules possible with a minimal volume of eluate and because it does not interfere with the filtration process, allowing for the preservation of the biological activity that separates the particles.