Waters, 186006852, ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 300 mm, 1/pk

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note: This column is also shipped with the BEH450 SEC Protein Standardat no additional cost at no additional cost (PN: 176002997) . Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: SEC
Separation Mode: SEC/GPC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 5000 psi (345 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 300 mm
Carbon Load: 9 %
eCord: Yes
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 300 mm, 100K - 1M, 1/pk The ACQUITY UPLC Protein BEH SEC Column uses the highly successful BEH particle technology and is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. Including the ACQUITY UPLC Protein BEH SEC Column, the total system solution will routinely deliver fast, accurate aggregate determinations of proteins up to 10 times quicker than traditional HPLC-based SEC methods. The patent-pending ACQUITY UPLC Protein BEH SEC Column set a new industry standard for protein analysis by being the first commercially available in the sub 2µm SEC chemistry. Using the ACQUITY UPLC Protein BEH SEC Column, you can deliver exceptional improvements in resolution, throughput, and sensitivity for assays. The lab equipment makes it possible for you to easily monitor the presence of protein aggregates through size-exclusion chromatography (SEC). As the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics, the ability to monitor the protein aggregates increases the efficacy of analysis. The ACQUITY UPLC Protein BEH SEC Column is ideally suited to work in conjunction with the ACQUITY UPLC System as its packing materials were designed specifically for this purpose. Additionally, the column is manufactured to the highest standards in a cGMP, ISO 9001 certified plant using ultra-pure reagents. To ensure that it continues to deliver outstanding performance, each batch of the packing material is tested chromatographically with acidic, basic, and neutral analytes. The results from these tests are held to narrow specification ranges to assure excellent, reproducible performance. Further, every ACQUITY UPLC Protein BEH SEC Column is individually tested, and a Performance Chromatogram and Certificate of Batch Analysis are provided on the eCord Intelligent Chip so you can rely on the quality of your lab equipment and can be certain that you will get consistent results. Shop for lab equipment and check all compatible products and other variants of the product listed here as well as our complete range of lab equipment from our website or catalog. You may also want to explore the BEH450 SEC Protein Standard Mix and other related products.

FAQ
How Is A Specific Protein Separated? In order to separate the protein from the cells where it is present, it is necessary to isolate the cells by centrifugation. In particular, centrifugation using media with different densities may be useful to isolate proteins expressed in specific cells.