Waters, 186006938, ACQUITY UPLC Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk < For superior peptide separations in LC and LC-MS applications, Waters’ synthesis process for Charged Surface Hybrid (CSH) particles offers a low-level positive charge to the surface of each particle. This CSH Technology allows columns to be used with standard TFA-containing eluents or weaker acid modifiers, meaning you no longer need to compromise and choose between a reversed-phase eluent’s sharp and symmetrical separated peaks and one that minimizes reduction of MS signal. With this chemistry, the ACQUITY UPLC Peptide CSH C18 Column gives you the ability to accept greater peptide mass loads than many other columns. With this power, you can detect potentially important love level constituents found within the major components of interest. Achieve comparatively excellent peak shape for peptides, using either FA- or TFA- containing mobile phases due to the 1.7µm particles in this column that contain a low and carefully defined concentration of positive charges. Because the performance of an ACQUITY UPLC Peptide CSH C18 column exhibits little dependence on strong ion-pairing agents, it is ideal for LC or LC-MS applications. CSH Technology lab equipment also facilitates improved sample mass loadability, delivering performance superior to many existing C18 columns on the market. With improved peptide mass loadability, these columns are excellent for challenging separations, especially those involving mixtures composed of species present at vastly different concentrations. Columns are easily employed for high peak capacity peptide separations using both UPLC and HPLC instrumentation. All Waters CSH C18 material is quality control tested with a cytochrome c tryptic digest in order to ensure column to column reproducibility. This process is performed using a gradient separation and 0.1% FA-containing eluents that challenge batch performance, helping to ensure consistent column performance for research and validated method requirements. Utilize Waters’ Cytochrome C Digestion Standard for optimal use of this column.

FAQ
What Is Peptide Separation? Peptide separation, in which peptides are purified, is one of the most time-consuming stages when determining the amino acid sequence of a protein. The method used for separation can vary based on molecular size, charge, polarity, solubility, and specific covalent or non-covalent interactions.
Reversed-phase HPLC is an essential tool for the separation and analysis of both proteins and peptides, often to purify proteins and peptides during studies. HPLC can be used well with mass spectrometry and other techniques relevant to the study of peptides or proteins and is a key piece in the separation of peptides from digested proteomes prior to protein identification by mass spectrometry.