Waters, 186006944, XSelect Peptide CSH C18 VanGuard Pre-column, 130Å, 2.5 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk

Waters CSH130 C18 based particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. Packed in ultra-low dispersion hardware, XSelect CSH130 C18 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption. The VanGuard pre-column connects directly to the inlet of an eXtended Performance column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Direct-Connect
Pore Size: 130 Å
QC Tested: Peptide
Format: VanGuard Pre-column
Surface Area: 185
System: UHPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 5 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: XSelect
Product Type: Guards
Units per Package: 3 pk

Additional Information
XSelect Peptide CSH C18 VanGuard Pre-column, 130Å, 2.5 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk XSelect CSH130 C18 2.5 µm eXtended Performance [XP] columns with ultra-low dispersion hardware provide exceptional separation performance, robustness, and throughput for HPLC assays while enabling a smooth transition path toward UPLC adoption. By removing superfluous connections, the VanGuard pre-column links directly to the intake of an eXtended Performance column, minimizing extra column volumes and leaks. Waters CSH130 C18 particles have a low and precisely defined concentration of positive charges, resulting in a comparatively excellent peak shape for peptides employing either FA- or TFA-containing mobile phases. Because the performance of a CSH130 C18 column is not overly dependent on strong ion pairing agents, it is appropriate for LC or LC-MS applications. The XSelect Peptide CSH C18 VanGuard Pre-column is based on Charged Surface Hybrid (CSH) Technology, which allows the column to overcome problems such as peak shape asymmetry or poor landing for basic chemicals, column bleed, and sluggish mobile-phase re-equilibrium. The CSH Technology provides various functional advantages to the XSelect Peptide CSH C18 VanGuard Pre-column, including superior peak shape for basic chemicals and high peak capacity for assessing complicated mixtures, which aids in the isolation and quantification of samples. The CSH Technology enhances many aspects of liquid chromatography in acidic, low ionic strength mobile phases, allowing the column to work well and provide similar backpressure. The Waters XSelect Peptide CSH C18 VanGuard Pre-column will assist your system in providing outstanding batch-to-batch repeatability, allowing you to swiftly design robust techniques and improve loading capacity and particle size scalability. With CSH Technology integrated into the XSelect Peptide CSH C18 VanGuard Pre-column, you can achieve a higher mass load for preparative separation without the use of ion-pairing chemicals. The XSelect Peptide CSH C18 VanGuard Pre-column allows for rapid column equilibration and the ability to swiftly change mobile-phase conditions and pH to screen numerous mobile-phase conditions. Browse our brochure or website to shop for lab equipment to meet all of your laboratory requirements. You may also be interested in the Cytochrome C Digestion Standard , which was created by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing grade trypsin. Protein digestion standards can be used for a variety of purposes, including HPLC and UPLC column evaluation and benchmarking.

FAQ
Under What pH and Pressure Conditions Can The XSelect Peptide CSH C18 VanGuard Pre-column Work? The XSelect Peptide CSH C18 VanGuard Pre-column offers a recommended operational pH range between 1 and 11 and has been designed to withstand a pressure of 18000 psi (or 1240 Bar); however, it is always recommended not to cross the maximum range to ensure the best possible column lifetime and effectiveness.