Waters, 186006957, XSelect Peptide CSH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk

Waters XSelect CSH130 C18 3.5 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: XSelect
Product Type: Columns
Units per Package: 1 pk

Additional Information
XSelect Peptide CSH C18 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1K - 15K, 1/pk Waters XSelect CSH130 C18 3.5 µm particles have a low and precisely defined positive charge concentration, resulting in a fairly good peak shape for peptides utilizing either FA or TFA-containing mobile phases. The low reliance on strong ion pairing agents in the performance of a CSH130 C18 column makes it appropriate for LC or LC-MS applications. The Waters Charged Surface Hybrid (CSH) technology enables the column to produce superior peak form and enhanced basic compound loading capacity. The CSH130 C18 chemistry's ability to absorb higher peptide mass loading than many other columns will improve the ability to detect potentially essential low-level constituents along with the main components of interest. Using either 0.1 percent FA or 0.1 percent TFA containing eluents, this column delivers remarkable peak capabilities in HPLC, UPLC, or LC-MS separations of peptides and small proteins up to 10,000 Daltons. You may achieve scalable chemistries with XSelect Peptide CSH C18 Column, from nanoflow analysis to lab-scale purification, as well as excellent mass loading of complicated peptide samples for peptide mapping, proteomics applications, or analysis and lab-scale purification of synthetic peptides. This particular analytical column is operational in the pH range of 1 to 11, up to a maximum pressure of 6000 psi (or 415 Bar), and has a Molecular Weight Range between 1,000 to 15,000. If you would like to review additional equipment offered by Waters, please review our brochure or visit our website to shop for lab equipment to meet the needs of your laboratory. You may also be interested in checking out LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk ; All Waters LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures. They are tested with mass spectrometers and come packaged with a certificate of analysis showing the reference and vial scan for the manufacturing lot.

FAQ
What Are The Best Recommended Techniques For Isolating And Cleaning Analytes? For separating and cleaning up sample components of interest, two general SPE methods are used. When the concentrations of the chemicals of interest are too low for accurate and precise quantification, a retention-cleanup-elution approach is typically used. This strategy allows for the concentration of dilute samples as well as trace enrichment of chemicals. When the desired sample component is present at a high concentration, a pass-through cleanup approach may be selected. When a pass-through cleanup approach is applied, however, no sample enrichment occurs.