Waters, 186007478, ACQUITY UPLC M-Class Peptide CSH C18 Column, 130Å, 1.7 µm, 75 µm X 250 mm, 1/pk

ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. This specific offering contains Waters patented CSH Technology 130 Å pore, C18, 1.7um particles. The CSH particle technology is built upon the hybrid BEH particle and contains a controlled low-level surface charge, providing enhanced selectivity and exceptional peak shape, particularly for basic compounds under low pH conditions in low-ionic strength mobile phases. The fact that CSH columns show little dependence on modifiers, such as formic acid, makes it ideal for LC-MS separations. In similar fashion, the ability of the CSH C18 chemistry to accept greater peptide mass loads enhances the ability to detect potentially important low level constituents contained with the major components of interest. The characteristic of the solid phase for fast column equilibration and pH switchover also helps increasing the sample throughput. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide Separation Column.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 10 pH
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Valco(male)|360um Open
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: Nano/Micro
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 75 µm
Length: 250 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: ACQUITY UPLC M-Class
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC M-Class Peptide CSH C18 Column, 130Å, 1.7 µm, 75 µm X 250 mm, 1/pk ACQUITY UPLC M-Class Columns are optimized for nano- to micro-scale liquid chromatographic separation for biomarker discovery, protein and peptide identification, and characterization. All M-Class columns are designed to operate at 15k psi (1,000 bar) and handle pressure fluctuations during injection extremely well, resulting in superior longevity. Waters' patented CSH Technology 130 pore, C18, 1.7um particles are used in this offering. The CSH particle technology is based on the hybrid BEH particle and has a controlled low-level surface charge, which provides enhanced selectivity and exceptional peak shape, particularly for basic compounds in low-ionic strength mobile phases at low pH. CSH columns are ideal for LC-MS separations due to their low reliance on modifiers such as formic acid. Similarly, the CSH C18 chemistry's ability to accept higher peptide mass loads improves the ability to detect potentially important low-level constituents present with the major components of interest. By choosing our tools, software, chemistries, and services, you have all the resources you require to complete any analysis successfully. Only Waters Quality Parts have undergone testing and certification to ensure consistent, optimal performance of your Waters systems, so they are compatible with your Waters instruments to the fullest extent possible. You can be confident of consistent instrument operation, accurate and reproducible results, and continued system compliance when you use Waters Quality Parts. The solid phase's ability to provide fast column equilibration and pH switchover also contribute to increased sample throughput. Before being accepted as a Peptide Separation Column, each batch is QC tested using a gradient separation of a tryptic digest of cytochrome c. Through the Waters online store on our website, you can shop for lab equipment with confidence that the items you need for your analyses are of the highest caliber. You may also be interested in the MassPREP Peptide Mixture ; A void volume marker and nine carefully selected peptides with a wide range of polarities and isoelectric points are included in the MassPREP™ peptide standard mixture. Under reversed-phase HPLC conditions commonly used for peptide separations, all peaks are easily resolved. The MassPREP™ Peptide Standard Mixture is useful for evaluating HPLC columns and peptide separation systems.

FAQ
How Are Ion Exchange Packings Conditioned? Any Ion Exchange Packings used for samples in nonpolar, organic solvents should first be conditioned with the sample solvent. However, when working with polar solvents, a water-miscible organic solvent should be used first, followed by an aqueous solution with the appropriate pH, organic solvent content, and salt concentration.