Waters, 186007953, HIC Protein Standard Mix

The HIC Protein Standard Mix is used to benchmark HIC columns, especially the Waters Protein-Pak Hi Res HIC Columns. It is recommended that this standard be used on a regular basis for evaluation of column and system performance ensuring confidence in results. It is also very valuable for method development and troubleshooting. This standard is uniquely formulated and optimized with a 6-component intact protein mixture to span a typical chromotographic range in this application. It contains 0.05 mg Ribonuclease A, 0.025 mg Cytochrome C, 0.05 mg Myoglobin, 0.03 mg Lysozyme, 0.1 mg Yeast Enolase and 0.05 mg of alpha-cymotrypsinogen A. It is packaged in a Waters TruView vial (ideal for peptides) as a lyophilized solid. Recommended solubilization is in 100 µl of distilled, purified Water or 50/50 mix of Mobile Phase A and B (low salt, pH 6.9). Store at -20 °C immediately upon arrival.

Specifications
Separation Mode: Reversed Phase
Detection Method: UV
Formulation: Solid
Storage Condition: -20C (Freezer)
System Type: LC
Shelf Life: 24 Months
Shelf life upon reconstitution: 1 Week
Shipping Condition: Ambient
Type: Reference
UNSPSC: 41116107
Application: Protein
Product Type: Standards
Units per Package: 1 pk

Additional Information
HIC Protein Standard Mix The HIC Protein Standard Mix is a valuable tool for hydrophobic interaction chromatography (HIC) applications. This product offers a mixture of six proteins with varying hydrophobicities, making them well-suited for assessing the performance of HIC columns. As a reference standard, the HIC Protein Standard Mix aids in the accurate quantification of proteins through HIC. Designed to streamline the evaluation of HIC columns, this standard mix simplifies the process of verifying column functionality and performance. By using the HIC Protein Standard Mix, researchers can ensure that their HIC columns and lab equipment are operating optimally, leading to reliable and consistent results in protein quantification. The inclusion of six proteins with different hydrophobic characteristics ensures comprehensive testing of the HIC column's interactions with a diverse set of analytes. This mix serves as a reliable benchmark for the separation and retention of proteins in HIC, enhancing the accuracy of quantification. You can also purchase the mix as a part of Protein-Pak Hi Res HIC, 2.5 µm, 4.6 mm x 35 mm with HIC Protein Standard Mix, 1/pk . The HIC Protein Standard Mix's stable formulation guarantees consistent and reproducible results across multiple experiments. Its user-friendly nature allows researchers to seamlessly incorporate it into their HIC workflow without any complicated setup or preparation. Whether researchers are validating new HIC columns or ensuring the performance of existing ones, the HIC Protein Standard Mix provides a time-saving and dependable solution. By regularly using this standard mix, scientists can maintain the integrity and reliability of their HIC experiments, ultimately leading to robust and high-quality protein analysis. The HIC Protein Standard Mix is an indispensable tool for researchers and laboratories working with hydrophobic interaction chromatography. With its diverse protein composition and straightforward application, this product offers an effective means of evaluating and validating HIC column performance, leading to accurate and consistent protein quantification results. By providing a reliable reference standard, the HIC Protein Standard Mix serves as a vital asset for those seeking to optimize their HIC workflows and achieve precise protein analysis.

FAQ
What is included in the HIC Protein Standard Mix? The HIC Protein Standard Mix contains six proteins that are commonly used as standards for hydrophobic interaction chromatography (HIC). The proteins in the mixture have a range of hydrophobicities, which makes them ideal for evaluating the performance of a HIC column.
The proteins in the mixture are:
Bovine Serum Albumin (BSA)
Ovalbumin
Carbonic Anhydrase
Myoglobin
Cytochrome C
Lysozyme