Waters, 186008007, ACQUITY UPLC M-Class HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk

ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.

This specific offering contains Waters patented HSS Technology 100 Å pore, T3, 1.8um particles. The mechanically tolerant High Strength Silica [HSS] particle technology was developed to compliment Waters Hybrid Particle Technology [BEH and CSH].  HSS allows for silica based LC separations under UPLC conditions. The particle technology provides the highest retentivity for polar compounds and metabolites while keeping in the retention between polar and hydrophobic analytes balanced.

The higher retention may result in enhanced sensitivity due to improved desolvation as polar analytes elute in a higher percentage organic mobile phase.

The T3 bonding utilizes a trifunctional C18 Alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility.

.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 1.8 µm
Endfitting Type: Valco(male)|360um Open
Pore Size: 100 Å
Format: Column
Surface Area: 230
System: Nano/Micro
Particle Technology: HSS
USP Classification: L1
Inner Diameter: 75 µm
Length: 200 mm
Carbon Load: 11 %
UNSPSC: 41115711
Brand: ACQUITY UPLC M-Class
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC M-Class HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk The ACQUITY UPLC M-Class Columns are the best in terms of sensitivity, resolution, and repeatability for biomarker discovery as well as protein identification and characterization. They are optimized for nano- to microscale liquid chromatographic separation. Due to the lab equipment's excellent ability to manage pressure variations during injection, it has a long lifespan. In order to achieve peak performance, it's essential to build the right connections to prevent peak tailing and inefficient operation. Reverse-phase liquid chromatography retains polar compounds and metabolites better thanks to the ACQUITY UPLC M-Class HSS T3 Column. With the aid of this lab equipment, analyzers can more readily explore the material's pore structure. This yields balanced retention of polar and hydrophobic molecules without the usage of ion-pair reagents. Using a single chromatographic approach, evaluate a number of different and complicated formulation compositions utilizing the ACQUITY UPLC M-Class HSS T3 Column. Good peak shapes and pH stability are made possible by the trifunctional binding of the ACQUITY UPLC M-Class HSS T3 Column to the HSS particle substrate. This has higher retention than hybrid-based C18 columns and is also available. With the exceptional qualities of the ACQUITY UPLC M-Class HSS T3 Column, you can be sure that your lab will be more productive and successful in meeting your goals in addition to meeting the challenges of the laboratory. You can create a complete lab equipment portfolio by exploring our website and getting to know all of our available products. Since its founding, Waters has been at the forefront of cutting-edge research, developing cutting-edge products and meeting all of your analytical needs. The ACQUITY UPLC M-Class HSS T3 Column also uses the Waters HSS (High Strength Silica) particle technology, which was created to withstand UPLC pressures using a mechanically tolerant, silica-based material. The HSS (High Strength Silica) particle technology from Waters uses a cutting-edge synthetic method to significantly increase silica's mechanical stability while maintaining pore volumes that are similar to those seen in HPLC silica-based materials. More retentivity is achieved compared to hybrid particle technology. The HSS is also a superior substrate for the production of stationary phases with various selectivities. You may also want to explore the MassPREP Enolase Digestion Standard . No standard protein, trypsin, or other hydrophilic components are left undigested in the protein digest produced by this procedure. Each batch of protein digestion standards is created under rigorous quality control guidelines to guarantee that you only get the best standards for your lab.

FAQ
What Is The pH Range Offered By The ACQUITY UPLC M-Class HSS T3 Column? The ACQUITY UPLC M-Class HSS T3 Column listed here can work well within the 2-8 pH range. What Are The Temperature Limits Of The ACQUITY UPLC M-Class HSS T3 Column? The ACQUITY UPLC M-Class HSS T3 Column can tolerate a maximum of 45 C temperature.