Waters, 186008471, ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 2.1 mm X 150 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume. Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis Note: If you are developing a new method, please consider our newer technology, ACQUITY Premier 250Å SEC Columns, for superior recovery, separation, and resolution.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 7000 psi (483 Bar)
Endcapped: No
Bonding Technology: SEC
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L33
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about ACQUITY UPLC Protein BEH SEC Column What type of biomolecular characterization is the ACQUITY UPLC Protein BEH SEC Column specifically designed for? The ACQUITY UPLC Protein BEH SEC Column is optimized for the separation of proteins and peptides based on size, making it suitable for characterizing protein complexes, aggregates, and fragments in detail. Can ACQUITY UPLC Protein BEH SEC Column be utilized for quantitative protein analysis? Yes, the ACQUITY UPLC Protein BEH SEC Column can be used for quantitative analysis as it provides consistent and reproducible retention times and peak areas, which are essential for quantitation. How does temperature control affect the performance of the ACQUITY UPLC Protein BEH SEC Column? Temperature control can significantly impact the performance of the ACQUITY UPLC Protein BEH SEC Column by influencing protein conformation and interaction with the stationary phase, thus affecting the resolution and reproducibility of separations. Is the ACQUITY UPLC Protein BEH SEC Column compatible with a wide range of mobile phases for flexibility in protein separation? Yes, the ACQUITY UPLC Protein BEH SEC Column is designed to be compatible with a variety of mobile phases, allowing for flexibility in optimizing protein separations according to specific research needs. Are salt gradients applicable when using the ACQUITY UPLC Protein BEH SEC Column for protein analysis? Typically, salt gradients are not used with the ACQUITY UPLC Protein BEH SEC Column as size exclusion chromatography relies on size-based separation, although certain conditions may warrant the use of salt to enhance protein recovery or stability.

FAQ
How Do I Troubleshoot My ACQUITY UPLC Protein BEH SEC Column? The first step is to compare the current column performance to performance when your column was last functioning properly. Functional tests with protein mixture may reveal subtle changes to surface chemistry that impact the application.
Common symptoms of change to the column may include an increase in pressure, or a loss of resolution paired with increased peak tailing that may be caused by microbial contamination.
Increased peak tailing can also be caused by a failure of a tubing connector or material build-up on the column inlet frit.