Waters, 186008802, nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pk

nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 100 µm X 100 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 300Å pore, C18, 1.7 µm particles. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for especially large peptides in proteomics, protein characterization, and peptide synthesis. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column. The columns are equipped with the ZenFit connector, an easy to use, fingertight, tool free liquid connection. This ensures reproducible, leak free connections. The fittings will fit into Waters V-Detail ports as well as into Rheodyne/Vici compatible valves. Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 10 pH
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: 10-32 for 1/16" male /360um open
Pore Size: 300 Å
QC Tested: Peptide
Format: Column
Surface Area: 90
System: Nano/Micro
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 100 µm
Length: 100 mm
Carbon Load: 12 %
UNSPSC: 41115711
Application: Peptide
Brand: nanoEase M/Z
Product Type: Columns
Units per Package: 1 pk

Additional Information
nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pk The nanoEase M/Z Peptide BEH C18 Column employs Waters' patented BEH Technology 130A pore, C18, 1.7 µm particles, which aids in achieving optimal performance by ensuring appropriate connections are created to eliminate peak tailing and wasteful operation. The nanoEase M/Z Peptide BEH C18 Column provides the best performance in nano- to micro-scale liquid chromatographic separation. The lab equipment delivers the highest sensitivity, resolution, and repeatability for biomarker discovery and protein identification and characterization. To ensure you receive only the highest quality lab equipment, each batch of the nanoEase M/Z Peptide BEH C18 Column is QC tested using a gradient separation of a tryptic digest of cytochrome c before being QC accepted as a Peptide separation column. The nanoEase M/Z Peptide BEH C18 packing materials are designed specifically for use with the ACQUITY UPLC System and manufactured in a cGMP, ISO 9001-certified facility using ultra-pure reagents. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are constrained to tight specification ranges to assure exceptional, consistent performance. Each column is analyzed separately, and an eCordTM Intelligent Chip is used to generate a Performance Chromatogram as well as a Certificate of Batch Analysis. All nanoLC columns are designed for use at operating pressures of up to 15 kpsi (1,000 bar) and tolerate pressure changes during injection exceedingly well, providing a superior lifetime. The columns include the ZenFit connector, which is a simple, fingertight, tool-free liquid connection that assures repeatable, leak-free connections. The fittings are compatible with Rheodyne/Vici valves as well as Waters V-Detail ports. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is simple to use, reusable, and fingertight, and can withstand 90°C pressures. This streamlines the procedure of connecting crucial nano- and microflow UPLC fluids. The nanoEase M/Z Peptide BEH C18 Column can significantly reduce flow-path-induced variability in chromatographic results. You can browse our website to shop for lab equipment that meets your specific demands. You may also be interested in TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk ; TruView LCMS Certified vials are manufactured using tightly controlled manufacturing processes and handling procedures and are tested for cleanliness using mass spectrometers and for low analyte adsorption using ACQUITY/MS/MS.

FAQ
How Does Polarity Affect Chromatographic Separation? Because polar molecules are more strongly attracted to polar solvents, they travel further when employing a polar solvent than when using a non-polar solvent. The distance traveled by the spots can be compared to the total distance traveled by the solvent, and measurements and comparisons can be performed.