Waters, 186009249, nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 300 µm X 150 mm, 1/pk

nanoEase M/Z HSS T3 Trap Column, 100Å, 1.8 µm, 300 µm X 150 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300 µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters HSS T3, 100Å pore, 1.8 µm particles and are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 15000 psi (1034 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 1.8 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
Format: Column
Surface Area: 230
System: Nano/Micro
Particle Technology: HSS
USP Classification: L1
Inner Diameter: 300 µm
Length: 150 mm
Carbon Load: 11 %
UNSPSC: 41115709
Brand: nanoEase M/Z
Product Type: Columns
Units per Package: 1 pk

Additional Information
nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 300 µm X 150 mm, 1/pk For the best results in liquid chromatographic separation at the nano- to microscale, rely on the nanoEase M/Z HSS T3 Column. The analytical column provides the highest level of sensitivity, resolution, and repeatability for the identification and characterization of proteins and the discovery of biomarkers. This particular lab equipment offers a 300µm ID and the same ease of use as more conventional columns and is constructed using an extremely low dispersion column design. It is made to endure high operating pressures of 15 kpsi and handle pressure changes during injection quite effectively. It offers superior column longevity as a result. Special care is given throughout the manufacturing process to produce the lab equipment to the highest standards since it is crucial to make sure that suitable connections are made to reduce peak tailing and inefficient operation in order to achieve optimal performance. Before being QC recognized as a Peptide separation column, each batch is QC evaluated utilizing a gradient separation of a tryptic digest of cytochrome c. ZenFit Connectors are pre-assembled on the nanoEase M/Z HSS T3 Column. The V-detail type connectors ports are where ZenFit Connectors are intended to be used. The 10-32 threaded port used for the V-detail connection may accommodate 1/16" O.D. tubing. These fingertight couplings are simple to use, reusable, and capable of withstanding high pressures. This makes crucial nano- and microflow UPLC fluid connections quite simple to create. The nanoEase M/Z HSS T3 Column significantly reduces the flow path causing variability in chromatographic findings. The nanoEase M/Z HSS T3 Column is the appropriate lab equipment because of all of these characteristics. You may also be interested in the MassPREP Peptide Mixture . A void volume marker and nine carefully chosen peptides with a wide variety of polarities and isoelectric points are included in the MassPREP peptide standard mixture. Under the reversed-phase HPLC conditions frequently used for peptide separations, all peaks are easily resolved. The MassPREP Peptide Standard Mixture is excellent for testing HPLC peptide separation systems and columns.

FAQ
Across What pH Range Does The nanoEase M/Z HSS T3 Column Operate? The nanoEase M/Z HSS T3 Column listed here is able to function across the 2-8 pH range. What Temperature Limits Does The nanoEase M/Z HSS T3 Column Adhere To? The Waters nanoEase M/Z HSS T3 Column listed here is able to tolerate a maximum of 45 C temperature.