Waters, 186010111, Tris CaCl2 Buffer Salts, pH 7.5, 4/pk

Tris CaCl2 Buffer Salts, pH 7.5 is provided as lyophilized salts of tris(hydroxymethyl)aminomethane (tris) hydrochloride and calcium chloride. These salts are packaged in a screw top amber glass bottle and intended to be dissolved with 7 mL water to prepare a pH 7.5 buffer comprised of 100 mM Tris HCl and 10 mM calcium chloride. Tris CaCl2 Buffer Salts pH 7.5 are intended for use in the preparation of enzymatic digests for a balance of completeness of digestion and minimized deamidation artifacts. This includes use with RapiZyme trypsin.

Specifications
Formulation: Solid
Storage Condition: 2-8C (Fridge)
Shelf Life: 24 Months
Shelf life upon reconstitution: 6 months
Shipping Condition: Ambient
UNSPSC: 41116105
Application: Reagent
Product Type: Reagents
Units per Package: 4 pk

Additional Information
Quick Prep Tris CaCl2 Buffer Salts, pH 7.5, 4/pk Quick Prep Tris CaCl2 Buffer Salts provide a reliable and consistent buffer solution for proteomics workflows requiring enzymatic digestion. Packaged as lyophilized salts of tris(hydroxymethyl)aminomethane hydrochloride and calcium chloride, each vial is designed for easy reconstitution with 7 mL of water. When dissolved, the buffer yields a pH 7.5 solution composed of 100 mM Tris HCl and 10 mM CaCl2, optimized for compatibility with RapiZyme trypsin and similar digestion protocols. This buffer is ideal for researchers requiring a high-purity solution that minimizes deamidation artifacts during protein digestion. With a 24-month shelf life (and 6 months after reconstitution), Tris CaCl2 Buffer Salts are trusted in both regulated and discovery-based environments. Refrigerate between 2–8°C. Ambient shipping included. Pair it with RapiZyme Trypsin for complete digestion workflows, or shop lab equipment to support your full proteomic analysis pipeline. What is the composition of the Tris CaCl2 Buffer after reconstitution? Each vial, when dissolved in 7 mL of water, provides a solution containing 100 mM Tris HCl and 10 mM calcium chloride at pH 7.5. How long does the buffer last once reconstituted? After reconstitution, the buffer is stable for up to 6 months when stored properly at 2–8°C in the original amber glass bottle. Why is calcium chloride included in this buffer? Calcium ions are essential for enzymatic activity, especially when using trypsin, helping to promote completeness of digestion and reduce unwanted deamidation.