Waters, PSS830615, Spherisorb ODS1 Column, 80Å, 5 µm, 4.6 mm X 250 mm, 1/pk

Spherisorb ODS1 columns are general purpose, silica-based, reversed-phase C18 columns. The ODS1 packing features low ligand density with a specified carbon load of 6.2%.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Bonding Technology: ODS1
Silanol Activity: High
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Parker-style
Pore Size: 80 Å
Format: Column
Surface Area: 220
System: HPLC
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 6 %
UNSPSC: 41115709
Brand: Spherisorb
Product Type: Columns
Units per Package: 1 pk

Additional Information
Spherisorb ODS1 Column, 80Å, 5 µm, 4.6 mm X 250 mm, 1/pk The Spherisorb ODS1 Column is a general-purpose, silica-based, reversed-phase C18 column and an excellent piece of lab equipment for your liquid chromatography needs. The packing of the Spherisorb ODS1 Column features low ligand density with a specified carbon load of 6%. This is critical as the way a column is packed will influence four very important performance features. These include peak symmetry, column efficiency, column lifetime, and column-to-column reproducibility. The column packing processes used by Waters are developed to ensure maximum performance at all times. It is crucial to understand the effect of all process parameters such as slurry solvent choice, packing pressure, time, temperature, flow rate, and slurry volume
in order to pack the best column. This is why Waters R&D engineers do extensive research to develop a packing process for each product we offer. This results in the lab equipment you can trust and rely upon to get the best peak symmetry, highest column efficiency, and best column stability. To guarantee you get the most reliable lab equipment, each Spherisorb ODS1 Column is individually tested, and a copy of the test chromatogram is supplied with each column. Explore the Spherisorb ODS1 Guard Cartridge, 80Å, 5 µm, 4.6 mm X 30 mm, 3/pk . The guard cartridge is designed specifically for the Spherisorb ODS1 Column, to ensure that your analytical column delivers high performance over a long period of time. This is achieved by removing any chemical or particulate contaminants from the mobile phase, and thus, protecting the column. To see other available products, related product ranges, or to see our complete inventory, peruse through our portfolio and shop for lab equipment .

FAQ
How Is Regeneration Carried Out For The Spherisorb ODS1 Column? To regenerate the Spherisorb ODS1 Column, pump 20-30 column volumes of dichloromethane and isopropanol through the column. Based on the suspected contamination, other solvents may also be used. Another recommended wash solvent for regeneration of the Spherisorb ODS1 Column is tetrahydrofuran (THF).

As determined by the sample contamination, you may need to replace guard columns at regular intervals as well. A sudden appearance of split peaks is an indication that the guard column needs replacement. Another factor indicating this is the increase in system backpressure above a set pressure limit.