Waters, WAT084040, µBondapak Amino (NH2) Column, 125Å, 10 µm, 3.9 mm X 300 mm, 1/pk

If your method calls for µBondapak packings, there is only one µBondapak packing material. Many companies claim to have columns with µBondapak-like selectivity but not one has ever passed Waters stringent QC batch test. Waters set the standard for reproducible, high-performance chromatographic columns. Waters µBondapak columns are still the most widely referenced and requested columns in the world.

Specifications
Chemistry: Amino (NH2)
Separation Mode: Normal-Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: High
Particle Shape: Irregular
Particle Size: 10 µm
Endfitting Type: Waters
Pore Size: 125 Å
Format: Column
Surface Area: 330
System: HPLC
USP Classification: L8
Inner Diameter: 3.9 mm
Length: 300 mm
Carbon Load: 4 %
UNSPSC: 41115709
Brand: µBondapak
Product Type: Columns
Units per Package: 1 pk

Additional Information
µBondapak Amino (NH2) Column, 125Å, 10 µm, 3.9 mm X 300 mm, 1/pk While many column manufacturers claim to have “µBondapak like selectivity” only Waters offers a column that contains the µBondapak C18 packing material, which is why no other column has ever passed our stringent quality control batch tests. µBondapak remains one of the most widely referenced and requested columns due to the year-to-year reproducibility achieved with this technology. This reversed-phase column sets the industry standard for reproducibility. Many HPLC packing materials were initially composed of non-spherical or irregularly shaped particles, which led to reduced mechanical stability and lower efficiency when compared to columns packed with spherical particles. Even with these challenges, many methods today continue to require the use of sorbents with irregularly shaped particles. For over 40 years, Waters has demonstrated a consistent and reliable column performance in our manufacturing of sorbents and bonded materials. We continue to support these columns and accessories like the µBondapak Amino (NH2) Guard-Pak Insert, 125Å, 10 µm, 10/pk . Like all Waters lab equipment , µBondapak Amino (NHS) Columns are manufactured using tightly controlled processes and narrow specification ranges to ensure high quality and reproducible results across chromatographic applications. This model offers a pore size of 125Å and a particle size of 10 µm and comes with one column per package. It is recommended for use at a pH range between 2 pH and 8 pH and up to a maximum pressure of 6,000 psi (415 bar).

FAQ
What Is The Proper Way to Clean µBondapak Amino (NH2) Columns? When a shift in retention or resolution occurs, it may mean contamination has occurred in the column. Usually flushing with an organic solvent is sufficient to remove the contaminant.
However, if system backpressure increases with usage, the guard column may need replacing if being used. If no guard column is in place, replacing the inlet frit can be the fix. If the problem continues, the column may be plugged with a contaminant that requires an appropriate solvent to dissolve the substance. For example, proteinaceous material can be removed using repeated 200 µL injections of dimethylsulfoxide.
If this flushing procedure still does not solve the problem, wash the column with a sequence of progressively more non-polar solvents. For example, you can switch from water to tetrahydrofuran (THF) to methylene chloride. Return to standard mobile phase conditions by reversing the sequence.