Agilent Biopharma HPLC Analysis

Agilent Biopharma HPLC Analysis solutions are engineered to meet the unique demands of biologics development and production. Whether you're analyzing monoclonal antibodies (mAbs), therapeutic proteins, peptides, or vaccines, Agilent offers a full suite of high-performance HPLC columns and consumables tailored for precise characterization and consistent quantification. With proven reliability and method robustness, these solutions support every stage from early research through GMP-compliant quality control, making Agilent a trusted partner in biopharma workflows.

Why Choose Agilent for Biopharma HPLC?

Biologics-Optimized Column Chemistry

Agilent columns are purpose-built for the complex nature of large molecules, offering unmatched resolution for proteins, peptides, aggregates, and glycans across various modes such as SEC, RP, IEX, and HILIC.

High Reproducibility and Batch Consistency

Precision manufacturing processes ensure minimal variation between lots—critical for regulated environments and reproducible CQA (Critical Quality Attribute) analysis.

Regulatory-Ready & 21 CFR Part 11 Support

Agilent HPLC systems and software solutions are designed for GLP/GMP labs, with built-in features to support data integrity, audit trails, and regulatory compliance.

Broad Compatibility

Biocompatible and bio-inert hardware ensures compatibility with all major LC systems, including Agilent’s own LC/MS, and other third-party instruments.

Method Development Tools & Application Kits

Extensive application notes, validated methods, and pre-configured kits streamline setup and accelerate productivity for new molecules and biosimilars.

Typical Applications in Biopharma Analysis

Monoclonal Antibody (mAb) Characterization
Includes SEC for aggregation detection, IEX for charge variant analysis, and RP for peptide mapping—critical steps in ensuring batch-to-batch consistency and product safety.

Peptide Mapping for Protein ID and Sequence Confirmation
Helps confirm amino acid sequences, detect post-translational modifications, and verify the integrity of therapeutic proteins. Often used with LC-MS systems.

Glycan Profiling and Sialic Acid Analysis
Glycosylation is a key quality attribute in mAbs and vaccines. Agilent HILIC columns enable fast and sensitive N-linked glycan separation, often within 15 minutes.

Host Cell Protein (HCP) and Residual Impurity Analysis
Specialized columns and workflows enable trace-level detection of process-related impurities, essential for regulatory compliance.

Biosimilar Development and Lot Comparability Studies
Analytical tools from Agilent assist in demonstrating molecular similarity, structural integrity, and process consistency across production lots or compared to reference drugs.

FAQs: Choosing the Right HPLC Tools for Biopharma

Q1. What is the best column for analyzing mAb aggregates?

The AdvanceBio SEC 300Å column provides high-resolution size exclusion chromatography, ideal for detecting aggregates and fragments in monoclonal antibodies.

Q2. Are Agilent columns compatible with non-Agilent HPLC systems?

Yes, most columns feature standard hardware fittings and are fully compatible with all major HPLC and UHPLC platforms.

Q3. Do you offer support for method development?

Agilent provides detailed application notes, validated protocols, and dedicated technical teams to assist in method setup and troubleshooting.

Q4. Are these products suitable for LC-MS workflows?

Absolutely. Columns such as AdvanceBio Peptide Mapping are designed with low-bleed, MS-compatible phases for clean baseline separation in LC-MS analysis.

Top-Rated Products for Biopharma HPLC Applications