Agilent HPLC Columns

Discover Agilent’s wide range of HPLC columns engineered for superior performance across pharmaceutical, environmental, food safety, and biopharmaceutical applications. Whether you're analyzing small molecules or complex biomolecules, Agilent offers reversed-phase, ion exchange, HILIC, and SEC columns to meet your specific separation needs. Experience high resolution, consistent batch performance, and full compatibility with all leading HPLC and UHPLC systems.

Key Benefits of HPLC Columns

• High-efficiency columns for accurate and reproducible separations
• Wide selection of bonded phases and particle sizes
• Compatible with Agilent and other major LC systems
• Ideal for small molecules, peptides, proteins, and more
• Backed by application notes and technical support

Agilent HPLC Columns Application

This part introduces some of the common application areas of Agilent HPLC Columns. 

Where Agilent HPLC Columns Excel

1. Pharmaceutical Development & Quality Control

Ideal for analyzing APIs, impurities, degradants, and excipients in accordance with regulatory guidelines (e.g., ICH, USP, EP).

Recommended columns:
- ZORBAX Eclipse Plus C18
- Poroshell 120 EC-C18
- InfinityLab Poroshell 120 SB-C18

2. Biopharmaceutical Characterization

Suitable for analyzing proteins, monoclonal antibodies, peptides, and oligonucleotides for purity, aggregation, and charge variants.

Recommended columns:
- AdvanceBio SEC
- AdvanceBio RP-mAb
- Bio IEX
- ZORBAX 300SB-C18

3. Food and Beverage Safety

Applied in detecting food additives, preservatives, dyes, pesticide residues, and contaminants in processed foods and beverages.

Recommended columns:
- ZORBAX SB-C18
- Poroshell 120 HILIC-Z
- Eclipse XDB-C18

4. Environmental Analysis

Used to quantify pollutants, drug residues, and organic compounds in water, soil, and air samples.

Recommended columns:
- Poroshell 120 EC-C18
- Eclipse Plus Phenyl-Hexyl
- ZORBAX Eclipse AAA

5. Forensic and Clinical Research

Supports sensitive and selective separation of drugs of abuse, metabolites, and biomarkers in biological matrices.

Recommended columns:
- Poroshell 120 SB-C18
- ZORBAX Eclipse AAA
- Poroshell 120 EC-C8

FAQs for HPLC Columns

Q1. How do I choose the right HPLC column?

Choose a column based on analyte type (e.g., small molecules, proteins), separation mode (e.g., reversed-phase, ion exchange, HILIC), resolution needs, and instrument compatibility. For general small molecules, use ZORBAX Eclipse Plus C18; for large biomolecules, choose AdvanceBio or Bio IEX series.

Q2. Are Agilent columns compatible with other LC systems?

Yes, Agilent columns are fully compatible with major LC systems including Waters, Shimadzu, Thermo Fisher, and others. Just ensure the dimensions and pressure rating match your system.

Q3. What precautions should I take when using and storing HPLC columns?

Always flush the column with suitable solvent before and after use to prevent buffer precipitation. Store reversed-phase columns in 50:50 water/acetonitrile or methanol. Never allow the column to dry out.

Q4. How do I know when to replace an HPLC column?

Signs like increased backpressure, longer retention times, reduced resolution, or peak tailing indicate the column may be worn out and should be replaced.

Popular Agilent HPLC Columns